Optimization of Extraction Process and Preliminary Component Identification of Phenolic Substances from Lonicerae flos
The natural dry Lonicerae flos was used as raw material.Ultrasonic-assisted ethanol solvent extraction method was adopted to optimize the extraction process of polyphenols from Lonicerae flos through a single factor experiment combined with the response surface method,and UPLC-MS/MS was utilized to conduct a preliminary qualitative and quantitative identification of its phenolic substances based on the plant's extensive target database.The results show that the optimal process conditions for the extraction of total phenols from Lonicerae flos are as follows:solid-liquid ratio of 10∶1 g/mL,ethanol concentration of 57%,ultrasonic power of 70 W,extraction temperature at 60℃,and extraction time of 11 min.The amount of extracted total phenols from Lonicerae flos is 71.08 mg/g;the phenolic acid of Lonicerae flos is mainly chlorogenic acid isomers,and the flavonoids are mainly tangerine,luteolin,and diosmin.
NETL
NSTL
万方数据
