Determination of Stachydrine Hydrochloride in Leonuri Herba from Different Manufacturers by HPLC-RID
A HPLC-RID method was established to determine the content of stachydrine hydrochloride in Leonuri Herba,and then the content of stachydrine hydrochloride in the Leonuri Herba samples from 14 manufacturers was determined.The results indicated that the content of stachydrine hydrochloride demonstrated a good linear relationship within the range of 0.100-0.523 mg/mL using the differential detector with the Mercury column(250 mm×4.6 mm,5 μm),mobile phase of 70:30 acetonitrile-water solution,flow rate of 1.0 mL/min,and column temperature of 45℃.The regression equation was y=91 519 x+1 342.2(R2=0.991 6),and the average recovery rate was 100.07%(RSD of 0.71%).The established HPLC-RID method was characterized by good repeatability,simple operation,rapid determination with stable results,and easy popularization.The content of stachydrine hydrochloride varied significantly among samples from different manufacturers,and only the samples from manufacturers 1367 and 8656 met the standards in the Chinese Pharmacopoeia.The content of stachydrine hydrochloride was associated with the medicinal characteristics of Leonuri Herba.According to the results,we suggested that users,pharmaceutical enterprises,and Chinese medicine manufacturers chose the Leonuri Herba materials with rich green leaves and fine stems.
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