湖南省猕猴桃叶片主要真菌病害的rDNA-ITS鉴定及序列分析
Identification of Pathogens Causing Major Fungal Diseases of Kiwifruit Leaves in Hunan Province by rDNA-ITS Sequencing
莫长安 1翟杨 2赵鑫 2潘峰 3杨媛茹 2汪洪鹰 2谭志坚 2曾粮斌2
作者信息
- 1. 桃江县植保植检站,湖南 益阳 413400
- 2. 中国农业科学院麻类研究所,湖南 长沙 410205
- 3. 永顺县农业农村局,湖南 永顺 416700
- 折叠
摘要
为了解湖南省猕猴桃叶部真菌病害病原菌的种类和相对多度,在长沙市、邵阳市、常德市、怀化市、张家界市和湘西土家族苗族自治州等地的猕猴桃种植区采集了'红阳''米良 1 号''翠玉'等 6 个猕猴桃品种的 31 份感病叶片样本,对病叶上的病原真菌进行分离、筛选和纯化.通过rDNA-ITS鉴定,结合UNITE数据库注释,统计分离出的病原真菌的种类和相对多度,比较了不同地区和不同寄主品种中的致病真菌的种类和相对多度差异.结果表明:31 份感病叶片样本中共分离出3 门 25 属 75 种 316 株病原真菌,以链格孢属、炭疽菌属和间座壳属为主要群体,分别占比 36.71%、18.04%和 14.56%;按照地区分布,菌属数量怀化市最多、其次是邵阳市和湘西州;按照猕猴桃品种分布,菌属数量排名前三的依次为'红阳''米良 1 号'和'翠玉'.
Abstract
This study aims to identify the species and reveal the relative abundance of fungal pathogens causing fungal diseases of kiwifruit leaves in Hunan Province.A total of 31 diseased leaf samples were collected from plants of six kiwifruit varieties including'Hongyang','Miliang No.1',and'Cuiyu'in the kiwifruit planting areas in Hunan Province,and the pathogens were isolated and screened.The species and relative abundance of pathogenic fungi were determined by rDNA-ITS sequencing combined with annotation against the UNITE database.The species and relative abundance of the pathogens were compared among different areas and host varieties.A total of 316 pathogenic fungal strains were isolated from the diseased leaves,belonging to 75 species,25 genera of 3 phyla.Alternaria,Colletotrichum,and Diaporthe were the dominant genera,with the relative abundance of 36.71%,18.04%,and 14.56%,respectively.The area with the most pathogen genera was Huaihua City,followed by Shaoyang City and Xiangxi Tujia and Miao Autonomous Prefecture.The top three kiwifruit varieties with the most pathogen genera were'Hongyang','Miliang No.1',and'Cuiyu'.
关键词
猕猴桃/真菌病害/病原菌/分类鉴定Key words
kiwifruit/fungal disease/pathogen/identification引用本文复制引用
基金项目
湖南省重点研发计划(2021NK2003)
中国农科院科技创新工程项目(ASTIP-IBFC07)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据