摘要
为了制备烟草花叶病毒(TMV)免疫诱抗剂,研究采用壳寡糖、高锰酸钾、内生真菌发酵液、紫外线、银杏叶提取物等试剂处理,筛选出TMV弱毒/无毒株系制备的最优方法,并在室内开展免疫激活效应.结果表明:紫外线、0.1%高锰酸钾溶液、内生真菌发酵液 16 倍浓缩液、100 μg/mL壳寡糖溶液对TMV表现出良好的钝化效果,钝化液对TMV有较高的抑制率.钝化TMV能引起烟草多酚氧化酶(PPO)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)、过氧化氢酶(CAT)5 种防御酶活性显著升高,从而提高烟株对TMV感染的抗性.烟苗和大田烟叶TMV防控试验也证明钝化TMV在烟叶生产中可以作为TMV免疫诱抗剂使用,提高烟株的TMV免疫力和抵抗力.
Abstract
To develop immunity inducers against tobacco mosaic virus(TMV),chitosan oligosaccharide,potassium permanganate(0.1%solution),endophytic fungi fermentation broth(16-fold concentrated solution),ultraviolet(UV)rays,Ginkgo biloba extract,and other reagents were employed to ascertain the optimal method for cultivating attenuated/nontoxic strains of TMV.Subsequently,the immunity-activating effect of the developed inducer was evaluated in controlled indoor conditions.The results showed that UV rays,0.1%potassium permanganate solution,16-fold concentrated solution of endophytic fungi fermentation broth,and 100 μg/mL chitosan oligosaccharide solution exhibited pronounced passivation effect on TMV,leading to a substantial reduction in TMV activity.Furthermore,the passivation of TMV significantly increased the activities of five defense enzymes,namely polyphenol oxidase(PPO),peroxidase(POD),superoxide dismutase(SOD),phenylalanine ammonia lyase(PAL)and catalase(CAT),thus improving the resistance of tobacco plants against TMV infection.The prevention and control experiments on TMV-infected tobacco seedlings and field-grown tobacco leaves proved that passivated TMV can be used as the immunity inducer in tobacco production to improve the immunity and resistance of tobacco plants against TMV.
基金项目
湖南省烟草公司永州市公司科技项目(YZ2020KJ05)
湖南省教育厅科学研究重点项目(21A0519)
湖南省自然科学基金(2023JJ50070)
湖南省自然科学基金(2022JJ30272)
国家科技期刊平台
NSTL
维普
万方数据