Development and Verification of Immunity Inducers Against Tobacco Mosaic Virus(TMV)
To develop immunity inducers against tobacco mosaic virus(TMV),chitosan oligosaccharide,potassium permanganate(0.1%solution),endophytic fungi fermentation broth(16-fold concentrated solution),ultraviolet(UV)rays,Ginkgo biloba extract,and other reagents were employed to ascertain the optimal method for cultivating attenuated/nontoxic strains of TMV.Subsequently,the immunity-activating effect of the developed inducer was evaluated in controlled indoor conditions.The results showed that UV rays,0.1%potassium permanganate solution,16-fold concentrated solution of endophytic fungi fermentation broth,and 100 μg/mL chitosan oligosaccharide solution exhibited pronounced passivation effect on TMV,leading to a substantial reduction in TMV activity.Furthermore,the passivation of TMV significantly increased the activities of five defense enzymes,namely polyphenol oxidase(PPO),peroxidase(POD),superoxide dismutase(SOD),phenylalanine ammonia lyase(PAL)and catalase(CAT),thus improving the resistance of tobacco plants against TMV infection.The prevention and control experiments on TMV-infected tobacco seedlings and field-grown tobacco leaves proved that passivated TMV can be used as the immunity inducer in tobacco production to improve the immunity and resistance of tobacco plants against TMV.
tobacco mosaic virus(TMV)immunity inducerpassivationdefense enzymeprevention and control effect
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