A Rapid Propagation System for Polygonatum cyrtonema in Hunan
This study aimed to establish a rapid propagation system for Polygonatum cyrtonema in Hunan.With the leaves,rhizomes,buds,and seeds as explants,21 media were designed based on the available studies,and the optimal media for germination,propagation,and rooting of P.cyrtonema were selected.The results indicated the fresh seeds harvested in the current year were the best explants,and those disinfected and cut longitudinally showcased good performance in tissue culture.The optimal medium for germination was B5+1.0 mg/L 6-BA+0.5 mg/L KT+30 g/L sucrose+6.0 g/L agar+2.0 mg/L GA3.The optimal medium for propagation was MS+4.0 mg/L 6-BA+0.2 mg/L NAA+30 g/L sucrose+6.5 g/L agar.The optimal medium for rooting was 1/2 MS+1.0 mg/L NAA+30 g/L sucrose+6.5 g/L agar.The findings lay a theoretical foundation for producing tissue culture seedlings and provide technical support for expanding the planting scale of P.cyrtonema in Hunan.
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