Screening and Application of a Phthalate Ester-Degrading Strain under Cadmium Stress
To enrich the phthalate ester(PAE)-degrading microbial resources under cadmium stress,we screened out an efficient di-(2-ethylhexyl)phthalate(DEHP)-degrading strain B-7 by using the inorganic salt liquid medium containing DEHP and cadmium from the strains preserved in our laboratory and studied the characteristics of the strain.Strain B-7 was Bacillus amyloliquefaciens with high biosafety and strong ability of degrading DEHP.The strain exerted the optimal degradation effect at 25-40℃and pH 6-8.When cultured in the inorganic salt medium with an initial DEHP concentration of 400 mg/L and cadmium concentration of 10 mmol/L for 4 days,the strain showcased the degradation rate of 93.1%for DEHP.Moreover,the strain demonstrated strong degradation effects on dimethyl phthalate(DMP),diethylphthalate(DEP),and dibutyl phthalate(DBP).In the soil contaminated with both cadmium and DEHP,strain B-7 coordinated with the indigenous microorganisms in soil to increase the degradation rate of DEHP.The results suggested that strain B-7 demonstrated a promising application prospect in the bioremediation of cadmium and PAE-contaminated soil.
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