"红阳"猕猴桃根际土壤细菌的分离与鉴定
Isolation and Identification of Bacteria from Rhizosphere Soil of the Kiwifruit Variety'Hongyang'
瞿昕怡 1彭清忠 1向小奇 1易浪波1
作者信息
- 1. 吉首大学生物资源与环境科学学院,湖南 吉首 416000
- 折叠
摘要
为探明健康与感病"红阳"猕猴桃根际土壤的细菌种群差异,从湘西州保靖县猕猴桃种植区采集健康与感病 2 种根际土壤样品,通过纯培养法分离细菌,基于细菌菌落形态特征和 16S rRNA基因序列的系统发育分析进行种属鉴定.从 2 种根际土壤样品中共分离获得 30 株细菌,经鉴定分属于链霉菌属、红球菌属、芽孢杆菌属、北里孢菌属和节杆菌属 5 个属;健康猕猴桃根际土壤中优势菌群为芽孢杆菌属和链霉菌属;感病猕猴桃根际土壤中主要是芽孢杆菌属细菌,且没有分离到北里孢菌属细菌.这表明健康与感病"红阳"猕猴桃根际土壤中细菌在种属水平存在差异,推测链霉菌属细菌中可能存在拮抗溃疡病的菌株.
Abstract
This study aims to reveal the differences of rhizosphere soil microorganisms between the healthy and diseased plants of the kiwifruit variety'Hongyang'.Rhizosphere soil samples of the healthy and diseased plants were collected from the kiwifruit planting area of Baojing County,Xiangxi Prefecture,and bacteria were isolated by the pure culture method.The isolates were identified by morphological observation of colonies and phylogenetic analysis based on 16S rRNA gene sequences.A total of 30 bacterial strains were isolated from two kinds of soil samples,and they were identified as Streptomyces,Rhodococcus,Bacillus,Kitasatospora,and Arthrobacter.Bacillus and Streptomyces were the dominant bacteria in the rhizosphere soil of healthy kiwifruit plants.Bacillus was dominant and Kitasatospora was not discovered in the rhizosphere soil of diseased kiwifruit plants.The results demonstrated differences of bacteria genera in the rhizosphere soil between healthy and diseased plants of the kiwifruit variety'Hongyang',which indicated that there might be antagonistic strains of Streptomyces against kiwifruit canker.
关键词
猕猴桃/根际土壤/可培养细菌/分离/鉴定Key words
kiwifruit/rhizosphere soil/culturable bacteria/isolation/identification引用本文复制引用
基金项目
湖南省重点研发计划(2022NK2055)
湖南省大学生创新训练计划项目(S202310531067)
出版年
2024
国家科技期刊平台
NSTL
万方数据