Identification and Bioinformatics Analysis of NAC Transcription Factor Family in Camellia drupifera Loureiro
NAC transcription factors are unique transcription factors in plants,which contain a highly conserved segment of specific structural domains at the N-terminus and have important functions in the processes of plant growth and development,stress response,and secondary metabolite biosynthesis.In this study,based on the information of the third-generation full-length transcriptome data of Camellia drupifera Loureiro(C.drupifera),51 NAC transcription factors were obtained by bioinformatics screening.In order to study the characteristics of these gene families,their physicochemical properties were analyzed,and multilinear comparison,protein phylogenetic trees,conserved motifs,gene structure,and subcellular localization were investigated.The structure of the secondary protein domains was predicted,and the protein phosphorylation sites were analyzed.The results show that NAC proteins differ in the number of amino acids,and the hydrophobicity between amino acid sequences is presented as hydrophilic proteins.Their protein structures have five conserved sub-structural domains.The phylogenetic tree analysis of the NAC transcription factors shows that 116 genes can be classified into 10 sub-populations.Part of the NAC proteins of C.drupifera is clustered as a single sub-population with specificity,and the other parts of the NAC proteins are fused with Arabidopsis thaliana and rice as a mixed sub-population.Meanwhile,all the NAC proteins have NAM superfamily structures.The subcellular localization shows that all 51 NAC proteins of C.drupifera are located in the nucleus,and the secondary structure is dominated by a randomly curled structure,with the structures of α-helix and β-rotor present.All the protein phosphorylation sites contain serine(Ser),threonine(Thr),and tyrosine(Tyr).The gene family characteristics of NAC transcription factors from C.drupifera provide a reference for the subsequent research on their gene functions.
万方数据
维普
