Tissue Culture for Rapid Propagation of Amorphophallus bulbifer
This study used the bulbs of Amorphophallus bulbifer as the explants in tissue culture for rapid propagation,aiming to provide technical support for the large-scale cultivation of this plant.The optimal medium for inducing emergence was MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+30 g/L sucrose+5 g/L agar,in which the emergence rate reached 100%.The optimal medium for inducing callus was MS+2.0 mg/L 6-BA+0.5 mg/L GA3+30 g/L sucrose+5 g/L agar,in which the rates of calluses differentiated from leaves and petioles were 99.17%and 92.50%,respectively.The optimal medium for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.4 mg/L NAA+25 g/L sucrose+5 g/L agar,in which the rates of adventitious buds differentiated from leaves and petioles were 94.17%and 65.83%,respectively,and 3 buds and 1.87 buds were differentiated from each callus,respectively.The optimal medium for rooting was 1/2 MS+0.4 mg/L NAA+0.2 mg/L IBA+0.5 g/L activated carbon+20 g/L sucrose+5 g/L agar,in which the rooting rate was 97.50%and the average root number and length were 3.0 and 3.0 cm,respectively.After 10 days of acclimatization,the survival rate of transplanted seedlings in the mixed matrix(topsoil∶crushed coconut fiber-containing nutrient soil∶black sandy soil=1∶2∶1,volume ratio)was 91.7%.
万方数据
维普
