This study used the bulbs of Amorphophallus bulbifer as the explants in tissue culture for rapid propagation,aiming to provide technical support for the large-scale cultivation of this plant.The optimal medium for inducing emergence was MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+30 g/L sucrose+5 g/L agar,in which the emergence rate reached 100%.The optimal medium for inducing callus was MS+2.0 mg/L 6-BA+0.5 mg/L GA3+30 g/L sucrose+5 g/L agar,in which the rates of calluses differentiated from leaves and petioles were 99.17%and 92.50%,respectively.The optimal medium for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.4 mg/L NAA+25 g/L sucrose+5 g/L agar,in which the rates of adventitious buds differentiated from leaves and petioles were 94.17%and 65.83%,respectively,and 3 buds and 1.87 buds were differentiated from each callus,respectively.The optimal medium for rooting was 1/2 MS+0.4 mg/L NAA+0.2 mg/L IBA+0.5 g/L activated carbon+20 g/L sucrose+5 g/L agar,in which the rooting rate was 97.50%and the average root number and length were 3.0 and 3.0 cm,respectively.After 10 days of acclimatization,the survival rate of transplanted seedlings in the mixed matrix(topsoil∶crushed coconut fiber-containing nutrient soil∶black sandy soil=1∶2∶1,volume ratio)was 91.7%.
维普
万方数据
