From the transcriptome sequencing data of cotton seedling roots under cadmium(Cd)stress,16 genes encoding superoxide dismutase(SOD)in cotton were identified and subjected to bioinformatics analysis.The results showed that the proteins encoded by the 16 SOD genes were non-secretory and hydrophilic and had the lengths of 127-333 aa,relative molecular weights of 13 048.69-35 974.31 Da,isoelectronic points of 4.43-8.50,and no transmembrane domain.Among the proteins,75%were acidic proteins and 69%were stable proteins.Most of the subcells were located in chloroplasts.The 16 SOD belonged to three subfamilies,namely,Fe-SOD,Mn-SOD,and Cu/Zn-SOD,each of which had its own conserved motifs.The Mn-SOD and Fe-SOD subfamilies had close genetic relationship,which indicated that they had similar biological functions.The identification and characterization of the SOD family members in cotton lay a foundation for probing into the molecular mechanism of SOD genes in response to Cd stress and provide genetic resources for breeding the cotton cultivars with Cd tolerance.
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