摘要
为探明造成养殖沙塘鳢肠道出血的病原菌G-2 的特性,对G-2 进行全基因组测序和分析.基因组组分分析和基因功能注释结果显示:G-2 的总基因数为 5 134 个,其中蛋白编码基因 4 977 个,非蛋白编码基因 157 个,非编码基因中tRNA基因 107 个,rRNA基因 42 个,sRNA基因 8 个;分别有 5 109、3 339、3 362、2 543 个基因在对应的Nr、SwissProt、COG、KEGG数据库中获得注释;毒力基因分析表明G-2 含有多种肠毒素和溶血素基因、InhA蛋白、磷脂酶及蛋白质毒素,解释了该菌造成养殖沙塘鳢肠道出血的原因.基于G-2 菌株的 16S rRNA序列信息构建系统进化树,确定其为苏云金芽孢杆菌.
Abstract
Whole genome sequencing and bioinformatics analysis were carried out to characterize the pathogenic strain G-2 causing gut bleeding of cultured Odontobutis potamophila.The results showed that the genome of strain G-2 contained 5 134 genes,including 4 977 coding genes and 157 non-coding genes(107 tRNA genes,42 rRNA genes,and 8 sRNA genes).In Nr,SwissProt,COG,and KEGG,5 109,3 339,3 362,and 2 543 genes,respectively,were annotated.Strain G-2 carried multiple enterotoxin and hemolysin genes and the genes encoding InhA protein,phospholipase,and protein toxins,which explained the gut bleeding caused by this strain.The phylogenetic tree built based on the 16S rRNA gene sequence identified strain G-2 as Bacillus thuringiensis.
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