Composition and Diversity of Root Fungi of Four Rhododendron lapponicum Species in Yanzigou,Garze Tibetan Autonomous Prefecture
In order to facilitate the introduction,domestication,and transplantation of Rhododendron lapponicum species in Yanzigou,Garze Tibetan Autonomous Prefecture,Sichuan Province,as well as explore the diversity of root microbiota under different habitat conditions,the root systems of four R.lapponicum species including R.lapponicum,R.nivale,R.aganniphum,and R.russatum,were collected to assess the composition and diversity of the root fungi of the alpine R.lapponicum types in this region through high-throughput sequencing and bioinformatics analysis.The results showed that a total of 746 973 effective sequences were obtained from the rhizosphere soil samples of the four alpine R.lapponicum species,which were then aggregated into 1 101 operational taxonomic units(OTUs).These fungi belonged to 6 phyla,27 classes,30 orders,30 families,and 30 genera,with Ascomycota and Basidiomycota being the dominant phyla,displaying significant differences in community abundance.At the genus level,there were notable differences in the predominant fungal genera,particularly the rhizosphere fungal community of R.aganniphum,which not only had the highest number of unique OTUs but also the greatest community abundance.The rhizosphere fungal community of R.nivale had the fewest unique OTUs,while R.lapponicum had the lowest community abundance.Overall,the diversity of rhizosphere fungi associated with the four R.lapponicum species in the study was significantly lower compared to lower-altitude areas in the same region,which was consistent with the trend of reduced fungal diversity at higher altitudes.
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