Analysis of DUS Testing Traits and Research on Testing Methods for New Varieties of the Lotus Genus
The traits involved in DUS testing of 108 germplasm resources of the lotus genus collected from 2016 to 2023 are analyzed,and DUS testing methods of the lotus genus are studied,so as to further improve the DUS testing level and promote the breeding innovation of the lotus genus.The results show that 5 of the 43 traits involved in the DUS testing guide of the lotus genus have new expression states,and 4 traits that are not included in the DUS testing guide of the lotus genus could distinguish varieties,which affect the accurate identification of new varieties.As for the influence of standard varieties on the DUS determination of lotus genus varieties,the absence of some standard varieties has affected the DUS determination of lotus genus varieties.In terms of the influence of different cultivation containers on the determination of DUS testing traits,small,medium and large plant type lotus genus root varieties can be planted in containers with the side length or diameter of 40 cm×40 cm,80 cm×80 cm,and 1~2 m,respectively,so as to test the full expression of underground stem traits.In terms of the influence of different testing quantity treatments on testing results,reducing the number of plants from 20 to 12 does not affect DUS testing results of lotus genus.Hence,some suggestions are put forward:revising the DUS testing guide of the lotus genus,formulating the testing operation manual and shooting technical regulations,building the database of known varieties and fingerprint of the lotus genus,and strengthening variety protection and DUS testing publicity.
lotus genusnew varietiesDUS testingtrait analysismethod study