首页|添加菜籽饼或菜籽油对植烟土壤差异代谢物的影响

添加菜籽饼或菜籽油对植烟土壤差异代谢物的影响

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为明确施用菜籽饼或菜籽油对植烟土壤代谢物的影响,采用拟靶向代谢组学技术,分析土壤差异代谢物的变化特征。结果表明:与未施用菜籽饼或菜籽油的对照土壤比较,施用菜籽饼筛选出43种土壤差异代谢物,施用菜籽油共筛选出9种。施用菜籽饼培养的土壤,土壤差异代谢物主要为谷氨酰胺、脯氨酸、丙氨酸、γ-氨基丁酸、亮氨酸、L-异亮氨酸、焦谷氨酸和赖氨酸 8 种氨基酸,上调表达 14。72~207。94 倍;施用菜籽油培养的土壤,土壤差异代谢物主要为油酸、油酸异构体、十六烯酸异构体、二十酸和海松酸 5 种有机酸,上调表达 1。73~225。97 倍,另有乳糖和松二糖 2 种糖类分别上调表达 3。12 和16。91倍。综合可知,施用菜籽饼增加土壤中氨基酸等高氮代谢物积累,施用菜籽油提高土壤中有机酸、糖等高碳代谢物积累。
Effects of Rapeseed Meal or Oil on Differential Metabolites in Tobacco-Planting Soil
Pseudo-targeted metabolomics was employed to identify the differential metabolites in soil,on the basis of which the effects of the application of rapeseed meal or oil on metabolites in tobacco-planting soil were examined.Compared with the soil without application of rapeseed meal or oil,the soil with application of rapeseed meal and oil showed 43 and 9 differential metabolites,respectively.The differential metabolites in the soil with the application of rapeseed meal were mainly glutamine,proline,alanine,γ-aminobutyric acid,leucine,L-isoleucine,pyroglutamic acid,and lysine,which were up-regulated by 14.72 to 207.94 folds.The differential metabolites in soil with the application of rapeseed oil mainly included oleic acid,oleic acid isomers,hexadecenoic acid isomers,eicosoic acid,and pimaric acid,which were up-regulated by 1.73 to 225.97 folds.In addition,lactose and turanose were up-regulated by 3.12 folds and 16.91 folds,respectively,in the soil treated with rapeseed oil.In conclusion,the application of rapeseed meal promoted the accumulation of high nitrogen-containing metabolites such as amino acids in the soil,while the application of rapeseed oil increased the accumulation of high carbon-containing metabolites such as organic acids and sugars in the soil.

tobacco-planting soilsoil culturerapeseed mealrapeseed oilsoil metabolite

龙勇、林叶春、蔡凯、艾永峰、李洪勋、陈代荣

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铜仁市烟草公司松桃县分公司,贵州 松桃 554100

贵州省烟草科学研究院,贵州 贵阳 550081

贵州省烟草公司铜仁市公司,贵州 铜仁 554300

植烟土壤 土壤培养 菜籽饼 菜籽油 土壤代谢物

2024

湖南农业科学
湖南省农业科学院 湖南省科技厅星火促进会 湖南农业大学

湖南农业科学

影响因子:0.415
ISSN:1006-060X
年,卷(期):2024.(9)