添加菜籽饼或菜籽油对植烟土壤差异代谢物的影响
Effects of Rapeseed Meal or Oil on Differential Metabolites in Tobacco-Planting Soil
龙勇 1林叶春 2蔡凯 2艾永峰 3李洪勋 2陈代荣3
作者信息
- 1. 铜仁市烟草公司松桃县分公司,贵州 松桃 554100
- 2. 贵州省烟草科学研究院,贵州 贵阳 550081
- 3. 贵州省烟草公司铜仁市公司,贵州 铜仁 554300
- 折叠
摘要
为明确施用菜籽饼或菜籽油对植烟土壤代谢物的影响,采用拟靶向代谢组学技术,分析土壤差异代谢物的变化特征.结果表明:与未施用菜籽饼或菜籽油的对照土壤比较,施用菜籽饼筛选出43种土壤差异代谢物,施用菜籽油共筛选出9种.施用菜籽饼培养的土壤,土壤差异代谢物主要为谷氨酰胺、脯氨酸、丙氨酸、γ-氨基丁酸、亮氨酸、L-异亮氨酸、焦谷氨酸和赖氨酸 8 种氨基酸,上调表达 14.72～207.94 倍;施用菜籽油培养的土壤,土壤差异代谢物主要为油酸、油酸异构体、十六烯酸异构体、二十酸和海松酸 5 种有机酸,上调表达 1.73～225.97 倍,另有乳糖和松二糖 2 种糖类分别上调表达 3.12 和16.91倍.综合可知,施用菜籽饼增加土壤中氨基酸等高氮代谢物积累,施用菜籽油提高土壤中有机酸、糖等高碳代谢物积累.
Abstract
Pseudo-targeted metabolomics was employed to identify the differential metabolites in soil,on the basis of which the effects of the application of rapeseed meal or oil on metabolites in tobacco-planting soil were examined.Compared with the soil without application of rapeseed meal or oil,the soil with application of rapeseed meal and oil showed 43 and 9 differential metabolites,respectively.The differential metabolites in the soil with the application of rapeseed meal were mainly glutamine,proline,alanine,γ-aminobutyric acid,leucine,L-isoleucine,pyroglutamic acid,and lysine,which were up-regulated by 14.72 to 207.94 folds.The differential metabolites in soil with the application of rapeseed oil mainly included oleic acid,oleic acid isomers,hexadecenoic acid isomers,eicosoic acid,and pimaric acid,which were up-regulated by 1.73 to 225.97 folds.In addition,lactose and turanose were up-regulated by 3.12 folds and 16.91 folds,respectively,in the soil treated with rapeseed oil.In conclusion,the application of rapeseed meal promoted the accumulation of high nitrogen-containing metabolites such as amino acids in the soil,while the application of rapeseed oil increased the accumulation of high carbon-containing metabolites such as organic acids and sugars in the soil.
关键词
植烟土壤/土壤培养/菜籽饼/菜籽油/土壤代谢物Key words
tobacco-planting soil/soil culture/rapeseed meal/rapeseed oil/soil metabolite引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据