Effects of Rapeseed Meal or Oil on Differential Metabolites in Tobacco-Planting Soil
Pseudo-targeted metabolomics was employed to identify the differential metabolites in soil,on the basis of which the effects of the application of rapeseed meal or oil on metabolites in tobacco-planting soil were examined.Compared with the soil without application of rapeseed meal or oil,the soil with application of rapeseed meal and oil showed 43 and 9 differential metabolites,respectively.The differential metabolites in the soil with the application of rapeseed meal were mainly glutamine,proline,alanine,γ-aminobutyric acid,leucine,L-isoleucine,pyroglutamic acid,and lysine,which were up-regulated by 14.72 to 207.94 folds.The differential metabolites in soil with the application of rapeseed oil mainly included oleic acid,oleic acid isomers,hexadecenoic acid isomers,eicosoic acid,and pimaric acid,which were up-regulated by 1.73 to 225.97 folds.In addition,lactose and turanose were up-regulated by 3.12 folds and 16.91 folds,respectively,in the soil treated with rapeseed oil.In conclusion,the application of rapeseed meal promoted the accumulation of high nitrogen-containing metabolites such as amino acids in the soil,while the application of rapeseed oil increased the accumulation of high carbon-containing metabolites such as organic acids and sugars in the soil.