不同大豆品种浸提液对稗草和苜蓿的除草活性
Extracts of Different Soybean Cultivars Inhibit Barnyard Grass and Alfalfa
李皓 1黄山 2柏振东 1韩进财 2严盛 2柏浩东 2李祖任 2金晨钟1
作者信息
- 1. 湖南人文科技学院,湖南 娄底 417000
- 2. 杂草生物学及安全防控湖南省重点实验室,湖南 长沙 410125
- 折叠
摘要
为探索大豆在种植过程中潜在的化感作用,研究了 6 个不同大豆品种浸提液对稗草和苜蓿的除草活性.采用培养皿滤纸法、土壤封闭法和茎叶喷雾法测定了其对稗草和苜蓿的化感作用,并挑选出除草活性最高的大豆品种浸提液进行除草活性物质分析.结果表明:种子萌发试验中,在 0.01 g/mL浓度下,2701 品种大豆乙醇浸提液对稗草和苜蓿的根长抑制率分别是 65.3%和 62.0%、对二者的芽长抑制率分别是 80.2%和 17.5%,湘 24 品种大豆乙醇浸提液对稗草和苜蓿的根长抑制率为79.8%和 70.9%、对二者的芽长抑制率分别是 46.0%和 35.6%;土壤封闭试验中,在 0.01 g/mL浓度下,湘 24 品种大豆乙醇浸提液对稗草和苜蓿的抑制率为 48.4%和 35.2%;茎叶喷雾试验中,在 0.01 g/mL浓度下,湘 24 品种大豆乙醇浸提液对稗草和苜蓿的抑制率为67.0%和93.6%,2701 品种大豆乙醇浸提液的抑制率为76.4%和93%;除草活性物质追踪结果表明黄酮类次生代谢物质在浸提液中含量相对较高.由此可见,湘 24、2701 品种大豆乙醇粗提液具有一定的除草活性.
Abstract
To explore the potential allelopathy of soybean,we examined the inhibitory effects of the extracts of six soybean cultivars on barnyard grass and alfalfa.The allelopathic effects of soybean extracts on barnyard grass and alfalfa were determined by the seed germination test,pre-emergence application,and post-emergence spraying.Furthermore,we analyzed the active components in the soybean cultivar extract with the highest herbicidal activity.The results of the seed germination test showed that 0.01 g/mL ethanol extract of the soybean cultivar'2701'reduced the root length by 65.3% and 62.0% and the bud length by 80.2% and 17.5% for barnyard grass and alfalfa,respectively.The ethanol extract of the soybean cultivar'Xiang 24'decreased the root length by 79.8% and 70.9% and the bud length by 46.0% and 35.6% for barnyard grass and alfalfa,respectively.The results of pre-emergence application showed that 0.01 g/mL ethanol extract of'Xiang 24'demonstrated the inhibition rates of 48.4% and 35.2% on barnyard grass and alfalfa,respectively.The results of post-emergence spraying showed that 0.01 g/mL ethanol extract of'Xiang 24'had the inhibition rates of 67.0% and 93.6% and that of'2701'presented the inhibition rates of 76.4% and 93% on barnyard grass and alfalfa,respectively.Flavonoids were the main active components for the herbicidal activity of soybean extract.The results indicated that the crude ethanol extracts of'Xiang 24'and'2701'had herbicidal activity.
关键词
稗草/化感作用/除草活性/LC/MS/大豆Key words
barnyard grass/allelopathy/herbicidal activity/LC/MS/soybean引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据