摘要
为快速高效检测西瓜种子纯度和判定种子真伪,研究对 236 份西瓜种子进行了DNA快速提取,并设计合成了 25 对SSR引物,通过PCR扩增与琼脂凝胶电泳技术筛选核心引物,对 52 份未知纯度西瓜种子进行鉴定.结果表明:筛选的 17 对核心引物(HY、XG1、XG2、XG3、XG4、XG5、XG7、XG9、XG10、XG11、XG13、XG14、XG15、XG17、XG19、XG22、XG23)多数多态性好,扩增条带清晰,可用于鉴定西瓜种子或植株的杂合度;利用筛选出的 6 组核心引物对未知纯度种子进行鉴定,发现其中 50 份西瓜种子为杂合子,2 份西瓜种子纯合度无法鉴定.
Abstract
This study aims to detect the purity and examine the authenticity of watermelon seeds in a rapid and efficient manner.DNA was extracted from 236 watermelon seed samples,and 25 pairs of SSR primers were designed and synthesized.The core primers were then screened by PCR amplification and agar gel electrophoresis and used to identify 52 watermelon seed samples with unknown genetic purity.The results showed that most of 17 pairs of core primers(HY,XG1,XG2,XG3,XG4,XG5,XG7,XG9,XG10,XG11,XG13,XG14,XG15,XG17,XG19,XG22,and XG23)were polymorphic,with clear amplification bands,and thus can be used to identify the heterozygosity of watermelon seeds or plants.Furthermore,selected six pairs of core primers were used for the identification of seeds with unknown genetic purity,which revealed that 50 watermelon seed samples were heterozygous and 2 samples could not be identified for homozygosity.
维普
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