Detection of Genetic Purity in Watermelon Seeds Based on SSR Markers
This study aims to detect the purity and examine the authenticity of watermelon seeds in a rapid and efficient manner.DNA was extracted from 236 watermelon seed samples,and 25 pairs of SSR primers were designed and synthesized.The core primers were then screened by PCR amplification and agar gel electrophoresis and used to identify 52 watermelon seed samples with unknown genetic purity.The results showed that most of 17 pairs of core primers(HY,XG1,XG2,XG3,XG4,XG5,XG7,XG9,XG10,XG11,XG13,XG14,XG15,XG17,XG19,XG22,and XG23)were polymorphic,with clear amplification bands,and thus can be used to identify the heterozygosity of watermelon seeds or plants.Furthermore,selected six pairs of core primers were used for the identification of seeds with unknown genetic purity,which revealed that 50 watermelon seed samples were heterozygous and 2 samples could not be identified for homozygosity.
watermelonSSR markersidentification of genetic purity
万方数据
维普
