摘要
为筛选对多种重金属具有较强抗性的根瘤菌,通过从重金属污染农田采集的花生根瘤中分离纯化,得到 34 株根瘤菌,对其进行系统进化分析并构建 16S rRNA基因系统发育树,以及对重金属(Cu2+、Ni2+、Cr6+、Zn2+)的抗性分析.结果显示,34 株根瘤菌中有 33 株属于根瘤菌属(Rhizobium),1 株属于中华根瘤菌属(Ensifer);对Cu2+具有较强抗性的菌株为HG6、HG13、HM13、HM23、HM52,对Ni2+具有较强抗性的菌株为HM13、HW23、HW45,对Cr6+具有较强抗性的菌株为HM14、HM21、HM23、HW2,对Zn2+的抗性较强的菌株为HG6、HM1、HM52、HW20;HG13对4种重金属均具有较好抗性.
Abstract
To screen out the rhizobia with strong resistance to heavy metals,we isolated 34 strains of rhizobia from peanut nodules in the heavy metal-contaminated farmland.A phylogenetic tree was built for these strains based on the 16S rRNA gene sequences.Furthermore,the resistance of these strains to heavy metals(Cu2+,Ni2+,Cr6+,and Zn2+)was evaluated.The results showed that 33 out of the 34 strains belonged to Rhizobium and 1 strain belonged to Ensifer.Strains HG6,HG13,HM13,HM23,and HM52 demonstrated strong resistance to Cu2+.Strains HM13,HW23,and HW45 showed strong resistance to Ni2+.Strains HM14,HM21,HM23,and HW2 had strong resistance to Cr6+.Strains HG6,HM1,HM52,and HW20 showcased strong resistance to Zn2+.Strain HG13 had strong resistance to all the four heavy metals.
基金项目
四川省基本科研业务项目(2022JDKY0022)
四川省自然科学基金项目(2023NSFSC0015)
野生植物四川省科技资源共享服务平台()
峨眉山生物多样性四川省野外科学观测研究站平台资助()
维普
万方数据