Codon Usage Bias of the Chloroplast Genome in Lagerstroemia indica'Ebony Glow'
This study aims to explore the characteristics as well as the codon usage pattern and influencing factors of the chloroplast genome in Lagerstroemia indica'Ebony Glow'.High-throughput sequencing was employed to sequence the genome of this plant.Forty-seven coding sequences were selected for the analysis of codon usage bias.The results revealed that the chloroplast genome of L.indica'Ebony Glow'exhibited a typical tetrad structure,with a total length of 152 174 bp and the average GC content of 37.6%.The effective number of codons(ENC)ranged from 35.73 to 61.00,and the codon adaptation index(CAI)ranged from 0.55 to 0.65,which indicated a weak preference for specific codons in the chloroplast genome of L.indica'Ebony Glow'.The genome contained 11 optimal codons(AAU,ACA,AGA,AGU,CAU,CGA,CUU,GGA,UAU,UUG,and UUU),which suggested a preference for ending with A/U.The neutral mapping demonstrated a linear regression slope of 0.261 6 and the ENC plotting showed a large difference between measured ENC and theoretical ENC,which suggested that natural selection was the primary factor influencing codon usage bias.
NETL
NSTL
万方数据
