This study aims to explore the characteristics as well as the codon usage pattern and influencing factors of the chloroplast genome in Lagerstroemia indica'Ebony Glow'.High-throughput sequencing was employed to sequence the genome of this plant.Forty-seven coding sequences were selected for the analysis of codon usage bias.The results revealed that the chloroplast genome of L.indica'Ebony Glow'exhibited a typical tetrad structure,with a total length of 152 174 bp and the average GC content of 37.6%.The effective number of codons(ENC)ranged from 35.73 to 61.00,and the codon adaptation index(CAI)ranged from 0.55 to 0.65,which indicated a weak preference for specific codons in the chloroplast genome of L.indica'Ebony Glow'.The genome contained 11 optimal codons(AAU,ACA,AGA,AGU,CAU,CGA,CUU,GGA,UAU,UUG,and UUU),which suggested a preference for ending with A/U.The neutral mapping demonstrated a linear regression slope of 0.261 6 and the ENC plotting showed a large difference between measured ENC and theoretical ENC,which suggested that natural selection was the primary factor influencing codon usage bias.
NETL
NSTL
万方数据
