Optimization of the Extraction of Total Flavonoids from Kaemptia Parviflora Using the Response Surface Methodology
This study employed response surface methodology(RSM)to optimize the extraction process of total flavonoids from Kaempferia parviflora.Based on single-factor experiments,the Plackett-Burman design was utilized to rank the significance of seven factors,and the top three factors were subsequently subjected to a steepest ascent test.Subsequently,a Box-Behnken design was applied to optimize these factors,and regression analysis was performed to identify the optimal extraction conditions.The optimal extraction parameters for total flavonoids were determined to be:ethanol volume fraction 40％,ultrasonic power 162 W,(NH4)2SO4 concentration 0.15 g/mL,ultrasonic time 30 min,ultrasonic temperature 40 ℃,liquid-to-solid ratio 20∶1(mL/g),and particle size 100 mesh.Under these optimal conditions,the extraction yield of total flavonoids from Kaem pferia parviflora was 1.254 0％,which closely matched the predicted value.
Kaempfera parvifloratotal flavonoidsresponse surface methodologyultrasound-assisted extractionaqueous two phase extraction
万方数据
维普
