湖州师范学院学报2024，Vol.46Issue(10) ：27-35.

基于响应曲面法优化小花山柰总黄酮提取工艺

Optimization of the Extraction of Total Flavonoids from Kaemptia Parviflora Using the Response Surface Methodology

张宪哲 李泽印 赵梦佳 沈欣辉 王继栋 齐欢
湖州师范学院学报2024，Vol.46Issue(10) ：27-35.
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  • 维普
  • 万方数据

基于响应曲面法优化小花山柰总黄酮提取工艺

Optimization of the Extraction of Total Flavonoids from Kaemptia Parviflora Using the Response Surface Methodology

张宪哲 1李泽印 1赵梦佳 1沈欣辉 1王继栋 2齐欢2
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作者信息

  • 1. 湖州师范学院生命科学学院,浙江湖州 313000
  • 2. 湖州师范学院生命科学学院,浙江湖州 313000;湖州师范学院浙江省媒介生物学与病原控制重点试验室,浙江湖州 313000
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摘要

采用响应曲面法(response surface methodology,RSM)优化小花山柰总黄酮的提取工艺.在单因素试验的基础上,采用Plackett-Burman设计对7种因素进行显著性排序,并对前3种因素进行爬坡试验.采用Box-Behnken设计进行试验和回归分析,得到的小花山柰总黄酮最佳提取工艺条件为:超声功率为162 W,硫酸铵质量浓度为0.15 g/mL,乙醇体积分数为40％,超声时间为30 min,超声温度为40 ℃,液料比为20∶1(mL/g),粉碎度为100目.在此条件下,小花山柰总黄酮的提取率为1.254 0％,与预测值接近.

Abstract

This study employed response surface methodology(RSM)to optimize the extraction process of total flavonoids from Kaempferia parviflora.Based on single-factor experiments,the Plackett-Burman design was utilized to rank the significance of seven factors,and the top three factors were subsequently subjected to a steepest ascent test.Subsequently,a Box-Behnken design was applied to optimize these factors,and regression analysis was performed to identify the optimal extraction conditions.The optimal extraction parameters for total flavonoids were determined to be:ethanol volume fraction 40％,ultrasonic power 162 W,(NH4)2SO4 concentration 0.15 g/mL,ultrasonic time 30 min,ultrasonic temperature 40 ℃,liquid-to-solid ratio 20∶1(mL/g),and particle size 100 mesh.Under these optimal conditions,the extraction yield of total flavonoids from Kaem pferia parviflora was 1.254 0％,which closely matched the predicted value.

关键词

小花山柰/总黄酮/响应面法/超声波辅助提取法/双水相提取法

Key words

Kaempfera parviflora/total flavonoids/response surface methodology/ultrasound-assisted extraction/aqueous two phase extraction

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出版年

2024
湖州师范学院学报
湖州师范学院

湖州师范学院学报

影响因子：0.301
ISSN：1009-1734
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