首页|可见-近红外二区双通道荧光成像系统

可见-近红外二区双通道荧光成像系统

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
本文基于光学手段,开发出了一套能够兼顾可见光与近红外二区(NIR-Ⅱ)窗口的双通道宏观成像系统,能够实时输出具有NIR-Ⅱ荧光信息的高质量明场图像,解决了常规的NIR-Ⅱ宏观成像系统提供的荧光图像与明场图像割裂较大的问题.实验中利用不同厚度的脂肪组织测试了吲哚菁绿的NIR-II窗口荧光信号分别在1 100～1 700 nm长通滤光片与1 300～1 700 nm长通滤光片下的抗散射能力与成像表现.接着利用所搭建的双通道宏观成像系统分别在小鼠以及大鼠模型上实现了淋巴结成像并模拟进行了淋巴结的切除手术,模拟了对腹腔淋巴结清扫的过程.最后,在大鼠模型上添加了不同厚度的生物脂肪组织,模拟实际手术中脂肪组织覆盖在淋巴结上的情况,观察双通道系统的穿透能力.可见近红外二区双通道荧光成像系统通过给操作者提供直观的影像信息,缩减手术时间、提高患者的预后,在临床手术导航中具有较大的应用潜力.
Visible-NIR-Ⅱ dual-channel fluorescence imaging system
A dual-channel macroscopic imaging system has been developed based on optical methods,which can simultaneously capture the visible light and near-infrared second window(NIR-Ⅱ)fluores-cence.It could provide high-quality bright-field real-time images with the NIR-Ⅱ fluorescence informa-tion,addressing the significant disparity issue between fluorescence and bright-field images in conven-tional NIR-Ⅱ macroscopic imaging systems.In the experiment,the anti-scattering capability and imag-ing performance of NIR-Ⅱ fluorescence signals of indocyanine green(ICG)were tested using different thicknesses of adipose tissues in the band of 1 100-1 700 nm and 1 300-1 700 nm respectively.Subse-quently,the dual-channel macroscopic imaging system was used to obtain lymph node images of mouse and rat models,simulating the lymph node resection surgery and mimicking the process of ab-dominal lymph node clearance.Finally,different thicknesses of biological adipose tissues were added to the rat model to simulate the presence of adipose tissues covering the lymph nodes during actual sur-gery,and the penetration capability of the dual-channel system was observed.The visible and near-infrared second window dual-channel fluorescence imaging system provided the intuitive visual infor-mation to the operator,reducing surgery time and improving the patient prognosis,and held great po-tential for application in clinical surgical navigation.

fluorescence imagingdouble-channel macroscopic imaging systemnear-infrared second windowclinical surgical navigation

刘雯、倪沪桅、钱骏

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浙江师范大学 浙江省光信息检测与显示技术研究重点实验室 物理与电子工程学院,浙江 金华 321004

浙江大学 现代光学仪器国家重点实验室 光及电磁波研究中心 光电科学与工程学院 先进光子学国际研究中心,浙江杭州 310058

荧光成像技术 双通道宏观成像系统 近红外二区 临床手术导航

国家自然科学基金国家自然科学基金中央高校基本科研业务费专项资金浙江省自然科学基金

61975172820018742020-KYY-511108-0007LQ21F050007

2024

10.11972/j.issn.1001-9014.2024.03.016

红外与毫米波学报
中国光学学会 中国科学院上海技术物理所

红外与毫米波学报

CSTPCD北大核心
影响因子：0.612
ISSN：1001-9014
年,卷(期)：2024.43(3)
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