Construction of complex bacteria and characteristics of their petroleum hydrocarbon degradation
The degradation performance of petroleum hydrocarbons and the ability to degrade four crude oil components were analyzed for the laboratory-preserved strains in order to obtain an efficient microbial consortium capable of degrading petroleum hydrocarbons.Four strains(A1,A3,Y3,Y4)were selected and classified through ITS and 16S rRNA molecular identification.The composition of the efficient consortium for degradation was optimized.The results reveal that A1 belong to the genus Acinetobacter,A3 belongs to the genus Bacillus,while Y3 and Y4 belong to the genus Candida.The degradation rates of petroleum hydrocarbons for A1,A3,Y3 and Y4 are 42.27％,40.95％,62.02％and 69.84％,respectively.Among them,Y4 exhibits the highest degradation performance for alkanes(65.71％)and aromatics(23.69％).A1 demonstrates the highest efficiency in degrading asphalt(22.74％),while Y3 exhibits the highest efficiency in degrading gum(21.22％).A3 displays balanced degradation capabilities for all components.The consortium consisting of A1,A3 and Y4 exhibits the highest degradation performance,with a significantly higher degradation rate of 83.11％compared to individual strains(1.19-2.03 times higher).The optimal inoculation ratio of A1,A3 and Y4 is determined to be 0.9∶0.9∶1.5,resulting in a degradation rate of 88.34％.This study successfully screens a new high-efficiency bacterial consortium for degrading petroleum hydrocarbons,providing important guidance for further research and application in petroleum pollution.
万方数据
维普
