Determination of gluconic acid-δ-lactone by high performance liquid chromatography
Gluconic acid-δ-lactone is often prepared by evaporation concentration from gluconic acid aqueous solution and contains a small amount of gluconic acid and gluconic acid-γ-lactone.In order to determine gluconic acid-δ-lactone in the product,a high-performance liquid chromatography method for the rapid separation and anylysis of gluconic acid-δ-lactone,gluconic acid and gluconic acid-γ-lactone was established and the conditions were optimized for the determination of gluconic acid-δ-lactone.The Titank Hilic column was chosen for the separation of gluconic acid-δ-lactone,gluconic acid and gluconic acid-γ-lactone.The mobile phase was 0.01 mol/L acetonitrile-phosphoric acid aqueous solution(volume ratio 90:10),the column temperature was 30° C,the flow rate was 1.0 mL/min,the detection wavelength was 205 nm,and the injection volume was 20 μL.The results show that gluconic acid-δ-lac tone can be well detected within 7 min.Gluconic acid-δ-lactone presentes a good linear relationship(r＞0.999)in the mass concentration range of 0.5-2.5 mg/mL,the detection limit is 0.002 mg/mL,the limit of quantification is 0.5 mg/mL,the average spiked recovery of the three levels is 98.8％-99.9％,and the relative standard deviation is 0.1％-0.4％(n=5).This method characterizes short analysis time,high sensitivity and good separation effect,and can be used to determine gluconic acid-δ-lactone,gluconic acid and gluconic acid-γ-lactone simultaneously.
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