化学工程2024,Vol.52Issue(9) :14-19.DOI:10.3969/j.issn.1005-9954.2024.09.003

单性木兰多糖的提取及其抗氧化稳定性能

Extraction and antioxidant stability of polysaccharide from Magnolia kwangsiensis Figlar & Noot.

郑燕菲 韦凤 韦金田
化学工程2024,Vol.52Issue(9) :14-19.DOI:10.3969/j.issn.1005-9954.2024.09.003

单性木兰多糖的提取及其抗氧化稳定性能

Extraction and antioxidant stability of polysaccharide from Magnolia kwangsiensis Figlar & Noot.

郑燕菲 1韦凤 1韦金田1
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作者信息

  • 1. 广西民族师范学院化学与生物工程学院,广西崇左 532200
  • 折叠

摘要

探索最优工艺对单性木兰多糖进行提取,为高效开发单性木兰多糖在食品、药品、化妆品等行业的应用提供参考依据.利用超声波辅助提取方法,结合单因素试验、响应面优化试验来确定最佳提取工艺.利用Fick第二定律研究单性木兰多糖提取过程的动力学模型,并对单性木兰多糖的体外抗氧化作用及稳定性展开实验研究.结果表明:在超声时间60 min、超声功率300 W、超声温度60 ℃、料液比1∶40 g/mL的条件下,多糖得率最大,达到3.68%;建立多糖提取动力学模型,推算相对萃余率、表观速率常数、半衰期等函数值;单性木兰多糖总抗氧化活性弱于Vc(抗坏血酸),对·OH(羟基自由基)清除作用的半抑制质量浓度PIC50为0.40mg/mL;单性木兰多糖使用或储存时可加入适量防腐剂苯甲酸钠,但不宜与柠檬酸和氯化钠共用.单性木兰中的多糖有望开发为功能食品及天然药物.

Abstract

For efficient extraction of polysaccharide from M.kwangsiensis(Magnolia kwangsiensis Figlar & Noot.),it is important to explore the optimal process for the best extraction,which can provide a reference for the efficient development of polysaccharide for applications in the food,pharmaceutical,cosmetic,and other industries.Ultrasonic assisted extraction was used to extract the polysaccharide.The optimal process conditions were determined through single factor tests and response surface tests.Based on Fick's second law,the extraction kinetic model was established.Antioxidant effect and stability of polysaccharide from M.kwangsiensis were studied.The results show that the polysaccharide yield rate is 3.68%at ultrasonic power 300 W and 60 ℃ for 60 min with material-liquid ratio 1∶40 g/mL.Based on the polysaccharide extraction kinetics model,the extraction rate constant,relative raffinate,and half-life are calculated.The total antioxidant activity of polysaccharide from M.kwangsiensis is weaker than that of Vc(ascorbic acid),and the ρIC50(50%inhibiting mass concentration)for·OH(hydroxyl radical)scavenging is 0.40 mg/mL.The polysaccharide from M.kwangsiensis can be used or stored with appropriate amount of sodium benzoate,and not be shared with citric acid and sodium chloride.The polysaccharide from M.kwangsiensis is expected to be developed as functional foods and natural medicines.

关键词

单性木兰/多糖/提取工艺/动力学/抗氧化/稳定性

Key words

Magnolia kwangsiensis Figlar & Noot./polysaccharide/extraction technology/kinetics/antioxidant/stability

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基金项目

广西自然科学基金资助项目(2020JJB120001)

广西民族师范学院高层次人才科研项目(2023SBNGCC003)

广西民族师范学院高层次人才科研项目(2022GCC001)

出版年

2024
化学工程
华陆工程科技有限责任公司

化学工程

CSTPCD北大核心
影响因子:0.438
ISSN:1005-9954
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