Hydrothermal acid-controlled protonation of kaempferol-3-O-rutinoside from mulberry leaves
KR(kaempferol-3-O-rutinoside)possesses high pharmacological value.The hydrothermal acid-controlled alcohol extraction method was employed to extract KR from mulberry leaves.The parameters for KR extraction were optimized,and a kinetic model was developed to investigate the mass transfer solubilization and leaching process of KR.The results reveal that the addition mass concentration of citric acid is 0.050 g/L,along with the mass volume ratio of mulberry leaves to 45％ethanol solution of 1∶20 g/mL,and after treatment in hydrothermal reactor at 80 ℃ for 40 min,2.00 g of mulberry leaves can be soluble with KR 16.09 mg/g.This is 40 times higher than the content achieved through hydrothermal leaching of KR in an automatic traditional Chinese medicine boiler(0.41 mg/g).SEM(scanning electron microscope)analysis demonstrates a significant increase in surface pores of mulberry leaves after the hydrothermal acid-controlled reaction.Furthermore,FT-IR(Fourier-transform infrared spectroscopy)indicats that the hydrothermal acid-controlled reaction facilitats the cleavage of C-O and C-H bonds in the mulberry leaves,and the dissolution of KR follows a first-order kinetic model with a dissolution rate of-3.45 min-1.These results provide a theoretical basis for the development of mulberry leaves with high value.
kaempferol-3-O-rutinosidemulberry leaveshydrothermal acid control reactionkinetics
万方数据
维普
