Optimization of extraction technology of total polysaccharides from Huangqi Gancao Tang
OBJECTIVE To optimize the extraction process of total polysaccharides from Huangqi Gancao Tang.METHODS The polysaccharide was extracted by water extraction and alcohol precipitation,and the extraction process of total polysaccharide in Huangqi Gancao Tang was optimized by single factor and orthogonal experiments.RESULTS The optimum extraction conditions of total polysaccharides in Huangqi Gancao Tang were as follows:extraction temperature was 80℃,the ratio of material to liquid was 1∶20(g∶mL),degreasing time was 6h,extraction time was 1h,and extraction for 3 times.CONCLUSION Under the optimum extraction conditions,the yield of total polysaccharides was 9.71%.The verification results show that the optimized extraction process has high stability and repeatability.
Huangqi Gancao Tangwater extraction and alcohol precipitationpolysaccharideextraction pro-cess