蒲公英总黄酮的提取工艺优化及抗氧化活性评价
Optimization of extraction process and evaluation of antioxidant activity of total flavonoids from Dandelion
刘思琳 1高原 1武晓艺 1徐笛 1陈子欣1
作者信息
- 1. 哈尔滨商业大学 药学院,黑龙江省预防与治疗老年性疾病的药物研究重点实验室, 黑龙江 哈尔滨 150076
- 折叠
摘要
本文采用乙醇浸提法从蒲公英中提取总黄酮,考察了乙醇浓度、提取温度、提取时间等因素对蒲公英总黄酮提取效果的影响.以总黄酮含量为指标,在单因素实验基础上采用正交实验法对提取工艺进行优化.最佳工艺条件为:乙醇浓度为 40%,提取温度为70℃,提取时间为80min,此条件下蒲公英提取物总黄酮含量为 0.9170mg·mL-1.通过DPPH清除实验、羟基自由基清除实验,对蒲公英总黄酮提取物进行抗氧化活性评价,其中DPPH自由基清除率IC50 值为 0.8492mg·mL-1,羟基自由基的清除能力IC50 值为 1.0717mg·mL-1.该结果表明,蒲公英总黄酮提取物具有良好的抗氧化清除自由基能力.
Abstract
Total flavonoids were extracted from dandelion by ethanol extraction.The effects of ethanol concentration,extraction temperature,extraction time and other factors on the extraction efficiency of total flavonoids were investigated.The extraction process was optimized by orthogonal experiment based on single factor experiment.The optimal conditions were ethanol concentration of 40%,extraction temperature of 70℃,extraction time of 80min,under these conditions,total flavonoids content of dandelion extract was 0.9170mg·mL-1.The antioxidant activity of total flavonoids from dandelion was evaluated by DPPH scavenging experiment and hydroxyl free radical scavenging experiment.The IC50 value of DPPH free radical scavenging was 0.8492mg·mL-1,and the IC50 value of hydroxyl free radical scavenging ability was 1.0717mg·mL-1.The results showed that the flavonoid extract of dandelion had good antioxidant ability in scavenging free radicals.
关键词
蒲公英/总黄酮/工艺优化/抗氧化Key words
Dandelion/total flavonoids/process optimization/oxidation resistance引用本文复制引用
基金项目
黑龙江省自然科学基金(LH2022H001)
黑龙江省重点研发计划指导类项目(GZ20210110)
国家级大学生创新创业训练计划(202210240007)
出版年
2024
NETL
NSTL
维普
万方数据