摘要
本文以亚麻籽油为原料油,α-亚麻酸为提取目标产物,采用单因素法研究了不同尿素溶液与脂肪酸体积比、包合温度、包合时间以及尿素-乙醇溶液浓度等对α-亚麻酸提取率的影响,优化了 α-亚麻酸的提取工艺.实验结果表明,当脂肪酸与尿素溶液体积比为1:45、尿素-乙醇溶液浓度为1mol·L-1、包合温度为-15℃、包合时间为24h时,α-亚麻酸提取率可达81％.并通过液相色谱对提纯的α-亚麻酸进行表征,α-亚麻酸在12.5min出峰,符合α-亚麻酸的出峰位置.
Abstract
In this paper,using flaxseed oil as the raw material oil,α-linolenic acid as the target product for extraction,the effects of different volume ratios of fatty acids to urea solution,concentration of urea ethanol solution,inclusion temperature,and inclusion time on α-linolenic acid extraction rate were investigated by using the single factor method to optimization the α-linolenic acid extraction process.The experimental results show that when the volume ratio of fatty acids to urea solution is 1∶45,the concentration of urea ethanol solution is 1 mol·L-1,the inclusion temperature is-15℃,and the inclusion time is 24 hour,the extraction rate of α-linolenic acid can reach 81％.Finally,the purified α-linolenic acid was characterized by liquid chromatography,and α-linolenic acid peaks at 12.5 minutes,which was consistent with the peak position of α-linolenic acid.
基金项目
深圳市科技计划(20201216104032003)
维普
万方数据