轴向核苷修饰硅酞菁的合成与光动力活性
Synthesis and photodynamic activity of silicon (Ⅳ) phthalocyanine axially modified with nucleoside derivatives
沈小敏1
作者信息
- 1. 福州大学 化学学院,福建 福州 350108
- 折叠
摘要
本文合成并表征了一种轴向核苷修饰硅酞菁,即二[5'-(2',3'-O-异丙基)-6-氯嘌呤核苷氧基]硅酞菁(SP1).通过紫外吸收光谱和荧光光谱研究表明,该化合物在N,N-二甲基甲酰胺和含 1%Cremophor EL水溶液中以单体形式存在.该化合物具有较高的单线态氧,具有显著的光动力抗癌活性,对人体肝癌细胞HepG2 的半致死浓度(IC50)低至 0.2×10-6 mol·L-1.荧光共聚焦显微镜研究显示,SP1可定位于细胞线粒体中.研究表明,该化合物SP1 是一种有发展潜力的抗癌光敏剂.
Abstract
A new silicon phthalocyanine axially di-substituted with 5'-(2',3'-O-isopropyl)-6-chlorine purine moieties(SP1)was prepared and characterized by 1H NMR and HR-MS.The electronic absorption and fluorescence emission spectra showed that this compound was essentially nonaggregated in N,N-dimethyformamide and 1%cremophor EL aqueous solution.SP1 also exhibited efficient singlet oxygen generation ability and a high photodynamic activity against human hepatoma HepG2 cells upon light irradiation,with an IC50 value down to 0.2×10-6mol·L-1.Fluorescence confocal microscopy study indicated that SP1 located preferentially in the mitochondria of the cells.All the results demonstrated that SP1 was highly potential as a new anti-cancer photosensitizer.
关键词
硅酞菁/核苷/光动力治疗/抗癌活性Key words
silicon phthalocyanine/nucleoside/photodynamic therapy/anticancer activity引用本文复制引用
基金项目
福建省中青年教师教育科研项目(JAT210004)
出版年
2024
NETL
NSTL
万方数据