Determination of isovitexin and isoscoparin in Qinghuo capsules by HPLC
OBJECTIVE To establish a HPLC method for the analysis of isovitexin and isoscoparin in Qinghuo capsules.METHODS HPLC column was Waters C18(250×4.6mm,5μm)with acetonitrile-0.2％phosphoric acid solution(13.5∶86.5)as flow equal elution,the flow rate of 1mL·min-1 and the wavelength for detection was 340nm.RESULT Isoexin and isoscoparin had a good linear relationship with peak area in the ranges of 0.0323μg to 0.9702μg and 0.0083μg to 0.2479μg,respectively(r=0.9999).The average recoveries were 101.59％and 98.79％,and RSD were 1.32％and 1.68％(n=9),respectively.RSD of precision,stability and repeatability test results were all less than 1.00％.CONCLUSION The established method is simple,stable and sensitive,and can be used for the quality control of Qinghuo capsules.
万方数据
维普
