OBJECTIVE To establish an HPLC method for simultaneous determination of two C21 steroids including kidjolanin and caudatin in Cynanchum allichii Wight.METHODS The analysis of this drug was performed on a 30℃thermostatic waters Sunfire C18 column(4.6mm×250mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid(43:57)flowing at 1.0mL·min-1,and the detection wavelength was set at 224 nm.RESULTS Kidjolanin and caudatin had a good linear relationship with peak area in the ranges of 0.0109 to 0.544mg·mL-1 and 0.0079 to 0.3955mg·mL-1,respectively(r≥0.999 6).The average recoveries were 99.82%and 99.80%,and RSD were 1.01%and 1.04%(n=6),respectively.RSD of precision,stability and repeatability test results were all less than 1.00%.CONCLUSION The established method is simple,accurate and stable,and can be used to evaluate the quality of the Cynanchum allichii Wight.
维普
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