Simultaneous determination of kidjolanin and caudatin in Cynanchum allichii Wight by HPLC
OBJECTIVE To establish an HPLC method for simultaneous determination of two C21 steroids including kidjolanin and caudatin in Cynanchum allichii Wight.METHODS The analysis of this drug was performed on a 30℃thermostatic waters Sunfire C18 column(4.6mm×250mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid(43:57)flowing at 1.0mL·min-1,and the detection wavelength was set at 224 nm.RESULTS Kidjolanin and caudatin had a good linear relationship with peak area in the ranges of 0.0109 to 0.544mg·mL-1 and 0.0079 to 0.3955mg·mL-1,respectively(r≥0.999 6).The average recoveries were 99.82%and 99.80%,and RSD were 1.01%and 1.04%(n=6),respectively.RSD of precision,stability and repeatability test results were all less than 1.00%.CONCLUSION The established method is simple,accurate and stable,and can be used to evaluate the quality of the Cynanchum allichii Wight.
万方数据
维普
