In this experiment,dried Oenothera biennis L.powder was used as raw material and ethanol was used as solvent to extract flavonoids.The flavonoids extraction amount under different test conditions was measured by the ultraviolet spectrophotometry method.The best process for extracting flavonoids from Oenothera biennis L.was selected by single factor experiment and the orthogonal experiment.It is concluded that the conditions required to achieve the maximum extraction of flavonoids are as follows:extraction temperature is 70℃,extraction time is 4 hours,material-liquid ratio is 1∶30 and ethanol concentration is 80%.The amount of flavonoids extraction is 31.62mg·g-1 under the optimum conditions.The results of this experiment provide scientific reference value for the application expansion of Oenothera biennis L.
维普
万方数据
