高效液相色谱法同时测定六堡茶中6种风味物质的含量
Simultaneous determination of six flavor ingredients in Liupao Tea by HPLC
胡王艳 1韦福广 1李卓蔚 2潘梦武1
作者信息
- 1. 贺州市检验检测中心,广西 贺州 542800
- 2. 贺州学院,广西 贺州 542899
- 折叠
摘要
建立高效液相色谱法同时测定六堡茶中没食子酸、表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、儿茶素没食子酸酯、表儿茶素、没食子儿茶素6 种风味物质的含量.在液料比为 20∶1(mL∶g)条件下,茶叶粉末置于体积分数为 60%乙醇溶液中,于 50℃、180W功率超声提取 20min后过滤,滤液经 0.22μm滤膜再次过滤后进行检测,以Thermo Scientific Acclaim 120 C18 色谱柱为固定相,不同体积比的 0.05%(体积分数)H3PO4溶液和乙腈混合液为流动相进行梯度洗脱,并在 270nm波长处采用外标法定量检测.结果表明,在 20min内可实现 6 种物质的有效分离,且在一定浓度范围内与相应的峰面积呈良好的线性关系(r>0.998).该方法对不同物质的加标回收率为 90.9%～98.8%,结果准确度较好,可为相关产品的质量评价提供参考.
Abstract
The HPLC method was established for simultaneous determination of six flavor ingredients including gallic acid(GA),gallic catechin gallic acid ester(EGCG),epicatechin gallic acid ester(ECG),catechins gallic acid ester(CG),epicatechin(EC)and gallic catechin(GC)in Liupao Tea.In the liquid-mass ratio condition of 20∶1(mL∶g),the tea powder sample was extracted in ethanol solution that volme fraction was 60%by ultrasound that power was 180W in 50℃ water bath for 20min,and subsequent filtration.The filtrate was filtered by 0.22μm filter membrane once again before sample analysis,Thermo Scientific Acclaim 120 C18 column was used as stationary phase and mobile phase gradient elution using mixture of 0.05%phosphoric acid-acetonitrile.The detection wavelength of 210nm was utilized to measure targets via external standard method.The results showed that six flavor ingredients could be separated within 20min,and showed a good linear relationship between definite mass concentrations of different ingredients and corresponding peak areas(r>0.998).The average recoveries for the targets were between 90.9%and 98.8%,which revealed it had better accuracy.It could provide a reference for the quality assessment of Liupao tea.
关键词
高效液相色谱法/六堡茶/风味物质/含量测定/儿茶素类化合物Key words
high performance liquid chromatography(HPLC)/Liupao Tea/flavor ingredient/content determi-nation/catechin compounds引用本文复制引用
出版年
2024
NETL
NSTL
万方数据