Optimization of preparation technology of ginseng selenium polysaccharide by star-point design-response surface methodology
OBJECTIVE To optimize the preparation process of selenium-treated ginseng polysaccharide.METHODS Aqueous purification and precipitation method was used to extract polysaccharide,and sodium selenite-nitrate method was used to selenium-treat polysaccharide.Response surface methodology was used to analyze the selenium-treatment of ginseng polysaccharide on the basis of single factor.The content of selenium was determined by ultraviolet spectrophotometry,and the binding of selenium with polysaccharide was verified by infrared spectroscopy.RESULTS When the temperature was 71℃,the time was 6.52h,and the mass of sodium selenite was 524mg,the selenium content of selenium-treated ginseng polysaccharide could reach 4.217mg·g-1,and the characteristic peak of selenium could be observed in infrared.CONCLUSION The obtained process conditions were stable and reliable,which could provid theoretical basis and experimental reference for the process conditions of selenium-treated ginseng polysaccharide.
NETL
NSTL
万方数据
