首页|HPLC法测定安普胶囊中红景天苷的含量

HPLC法测定安普胶囊中红景天苷的含量

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目的 建立在安普胶囊中以红景天苷为指标性成分的含量的测定方法.方法 选用甲醇-水(15∶85)为流动相,流速为1.0mL·min-1,检测波长为 275nm,柱温为 30℃.结果 确定了安普胶囊的含量测定方法,在色谱条件下红景天苷回归方程为y=33.998x+2.9436(R2=0.9998),表明红景天苷在 0.5~5mg·mL-1 浓度范围内线性关系良好.结论 该方法简便、快捷,重现性好,能够满足实际质量控制的要求,可以用于安普胶囊中红景天苷含量的测定.
Determination of salidroside in Anpu capsules by HPLC
OBJECTIVE To establish a method for determining the content of salidroside as an indicator component in Anpu capsules.METHODS Methanol water(15∶85)was used as the mobile phase,with a flow rate of 1.0mL·min-1 and a detection wavelength of 275nm,the column temperature of 30℃.RESULTS The content determination method for Anpu capsules was determined,under chromatographic conditions,the regression equation for salidroside was y=33.998x+2.9436(R2=0.9998),indicating a good linear relationship between salidroside in the range of 0.5~5mg·mL-1.CONCLUSION This method is simple,fast,with good reproducibility,and can meet the requirements of actual quality control.It can be used for the determination of salidroside in Anpu capsules.

Anpu capsulessalidrosideHPLCcontent determination

何嘉郡、董晏含、张轶男、谢海龙、马伟

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黑龙江中医药大学,黑龙江 哈尔滨 150040

安普胶囊 红景天苷 高效液相色谱法 含量测定

黑龙江省自然科学基金联合引导项目黑龙江省博士后资助项目

LH2022H087LBH-Z20096

2024

化学工程师
黑龙江省化工研究院

化学工程师

CSTPCD
影响因子:0.243
ISSN:1002-1124
年,卷(期):2024.38(7)