Determination of salidroside in Anpu capsules by HPLC
OBJECTIVE To establish a method for determining the content of salidroside as an indicator component in Anpu capsules.METHODS Methanol water(15∶85)was used as the mobile phase,with a flow rate of 1.0mL·min-1 and a detection wavelength of 275nm,the column temperature of 30℃.RESULTS The content determination method for Anpu capsules was determined,under chromatographic conditions,the regression equation for salidroside was y=33.998x+2.9436(R2=0.9998),indicating a good linear relationship between salidroside in the range of 0.5~5mg·mL-1.CONCLUSION This method is simple,fast,with good reproducibility,and can meet the requirements of actual quality control.It can be used for the determination of salidroside in Anpu capsules.