首页|雪莲口服液药渣残留化学成分中总多糖和总多酚的含量测定

雪莲口服液药渣残留化学成分中总多糖和总多酚的含量测定

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本研究通过预实验证明了雪莲口服液药渣中残有多糖及多酚类物质,为了充分利用这两种活性物质,采用水提醇沉的方法,于雪莲口服液药渣中得到粗多糖,用苯酚-硫酸法进行含量测定;采用超声提取法提取多酚类物质,并用Folin-酚法对其进行含量测定.结果表明,多糖和多酚在2.5~17.5μg·mL-1浓度范围内均与吸光度呈线性关系,其平均含量分别为21.40%、0.089%,RSD分别为1.85%、2.42%,作为二次利用产物,雪莲口服液药渣中总多糖和总多酚类物质含量较高,有较大的利用价值,可作进一步研究.
Determination of total polysaccharides and total polyphenols in the residual chemical components of Snow lotus oral liquid residue
This study demonstrated through preliminary experiments that there are residual polysaccharides and polyphenols in the Snow lotus oral liquid residue,in order to fully utilize these two active substances,using the method of water extraction and alcohol precipitation,crude polysaccharides were obtained from Snow lotus oral liquid residue,and the content was determined using the phenol sulfuric acid method;Using ultrasonic extraction method,extract polyphenols and determine their content using Folin phenol method.The results showed that there was a good linear relationship between the absorbance and the concentration of polysaccharide in the range of 2.5~17.5μg·mL-1,and the average content of polysaccharide was 21.40%,RSD was 1.85%;Polyphenols showed a good linear relationship with absorbance in the concentration range of 2.5~17.5μg·mL-1with an average value of 0.089%and RSD of 2.42%.As a product of secondary utilization,the content of total polysaccharides and total polyphenols in the Snow lotus oral liquid residue is relatively high,which has great utilization value and can be further studied.

Snow lotus oral liquid residuecontent determinationpre-testpolysaccharidepolyphenol

李琦、王转梅、李森、王新宇、田树革

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新疆医科大学中医学院,新疆乌鲁木齐 830017

新疆天山雪莲制药有限公司,新疆阜康 831500

雪莲口服液药渣 含量测定 预实验 多糖 多酚

国家级大学生创新创业训练计划新疆维吾尔自治区科技重大专项

202210760032017A3005-2

2024

化学工程师
黑龙江省化工研究院

化学工程师

CSTPCD
影响因子:0.243
ISSN:1002-1124
年,卷(期):2024.38(8)
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