Determination of eight tetracyline antibiotics in phormaceutical wastewater by SPE-UFLC-MS method
A solid-phase extraction ultra high speed liquid chromatography-mass spectrometry(SPE-UFLC-MS)method was established for the determination of eight tetracycline antibiotics in pharmaceutical wastewater.By optimizing the conditions of solid-phase extraction,a solid-phase Oasis HLB extraction column,a purification agent of 30％zinc sulfate+20％potassium ferrocyanide solution,a stabilizer of 1g·L-1Na2EDTA,and an extraction pH value of 8 were selected to obtain the optimal extraction recovery rate of the target compound.The standard curves of eight tetracycline antibiotics have a good linear relationship(r＞0.999)and the detection limit is less than 10ng·L-1 The average recovery rate is 95.3％～106.9％,and the relative standard deviation(RSD)is less than 8.23％.The method has good precision and accuracy.After actual sample testing,except for dexamethasone hydrochloride and dimethylamine tetracycline hydrochloride,the other 6 tetracycline antibiotics were detected to varying degrees in the conditioning tank of pharmaceutical wastewater.This can provide reference for the detection of antibiotic compounds in pharmaceutical wastewater.
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