OBJECTIVE To establish fingerprints of Evodiae Fructus by HPLC,determine the content of evodiamine,rutaecarpine,evocarpine three alkaloids,and the quality of Evodiae Fructus from different producing companies was evaluated by cluster analysis.METHODS To draw 11 batches Evodiae Fructus of fingerprints by HPLC with evodiamine as the reference,followed by similarity evaluation and cluster analysis;HPLC was used to determine the content of three alkaloid components.RESULTS The HPLC fingerprint of Fructus Evodiae was established with 11 common peaks identified and 4 peaks identified.The similarity with the control fingerprint ranged from 0.898～0.996.The types and quantities of alkaloids in Evodiae Fructus from different regions were basically the same,and the content of the three alkaloids fluctuated significantly within a large range.11 batches of samples can be grouped into 3 categories.CONCLUSION This method is simple,reliable,and has good repeatability,and can be effectively and comprehensively used for the quality evaluation of Evodiae Fructus.
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