Study on extraction and properties of polys accharides and total phenolics from Wenshan Panax notoginseng
The polysaccharides and total phenolics in Wenshan Panax notoginseng were extracted by ultrasonic-assisted method.The extraction conditions were optimized based on single factor experiment,the moisture retention of polysaccharides and antioxidant activity of total phenolics from wenshan panax notoginseng were also determined.The results showed that the optimum extraction conditions of polysaccharides from Wenshan Panax notoginseng were as follows:the ratio of material to liquid of 1∶20,extraction time of 40min,extraction temperature of 60℃,then the extraction rate of polysaccharides was 7.875%.The moisture retention of polysaccharides from Wenshan Panax notoginseng was better than that of sodium alginate and glycerin.The optimum extraction conditions of total phenolics from Wenshan Panax notoginseng were as follows:the extraction temperature of 35℃,ratio of material to liquid of 1∶25,ethanol concentration of 82%,extraction time of 60min,then the extraction rate of total phenolics was 2.836%.The IC50 of total phenolics from Wenshan Panax notoginseng on DPPH·and ABTS·radicals were 6.607 and 10.623 μg·mL-1,respectively.