Study on the extraction process and antioxidant properties of Codonop sis Pilosula polysaccharide
To study the optimum conditions of extraction of polysaccharides from Codonop sis Pilosula,a single factor experiment was conducted using the underground part of three-year-old Codonop sis Pilosula as the raw material,and extraction temperature,time and liquid-solid ratio were designed.Based on this,a three factor three-level orthogonal expriment was conducted to optimize the extraction process,and the antioxidant activities of the polysaccharides were studied.The results showed that the optimum extraction conditions were obtained as follows:extraction temperature of 90℃,extraction time of 40min,solid-liquid ratio of 1∶60(g∶mL).Under these conditions,the extraction yields of Codonopsis Pilosula polysaccharides were 24.61％.In a certain concentration range,the scavenging ability increased with the increase of the polysaccharides concentration and showed a good correlation between the total scavenging ability and the concentration.The scavenged ability of the polysaccharides and VC on DPPH were similar at 12mg·mL-1 and the scavenging rates of polysaecharides and VC were 96.39％and 100％,respectively.The extraction rate of stinky ginseng polysacharides was hign and antioxidant activity was significant,which provides a basis for the development and utilization of the polysaccharide in the later period.
Codonops is Pilosulaorthogonal experimentpolysaccharideantioxidant activityhot water extraction
万方数据
维普
