To study the optimum conditions of extraction of polysaccharides from Codonop sis Pilosula,a single factor experiment was conducted using the underground part of three-year-old Codonop sis Pilosula as the raw material,and extraction temperature,time and liquid-solid ratio were designed.Based on this,a three factor three-level orthogonal expriment was conducted to optimize the extraction process,and the antioxidant activities of the polysaccharides were studied.The results showed that the optimum extraction conditions were obtained as follows:extraction temperature of 90℃,extraction time of 40min,solid-liquid ratio of 1∶60(g∶mL).Under these conditions,the extraction yields of Codonopsis Pilosula polysaccharides were 24.61％.In a certain concentration range,the scavenging ability increased with the increase of the polysaccharides concentration and showed a good correlation between the total scavenging ability and the concentration.The scavenged ability of the polysaccharides and VC on DPPH were similar at 12mg·mL-1 and the scavenging rates of polysaecharides and VC were 96.39％and 100％,respectively.The extraction rate of stinky ginseng polysacharides was hign and antioxidant activity was significant,which provides a basis for the development and utilization of the polysaccharide in the later period.
Codonops is Pilosulaorthogonal experimentpolysaccharideantioxidant activityhot water extraction
维普
万方数据
