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UPLC法同时测定忍冬藤胶囊中9种有效成分的含量

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目的建立超高效液相色谱法(UPLC)同时测定忍冬藤胶囊中9种有效成分的含量。方法采用Shim-pack GIST C18色谱柱,流动相:乙腈和0。04%H3PO4水溶液,梯度洗脱,流速:0。4mL·min-1,检测波长:327nm和240nm,柱温:25℃,进样量:10μL。结果9种有效成分线性关系、精密度、稳定性均符合方法学测定要求,平均回收率为101。3%~102。6%。结论该法简单、准确可行,可为忍冬藤胶囊的质量控制提供依据。
Simultaneous determination of nine active components in Rendongteng Capsules by UPLC
OBJECTIVE To establish a UPLC method for the simultaneous determination of nine active components in Rendongteng Capsules.METHODS Shim-pack GIST C18 colum,mobile phase of acetonitrile:0.04%phosphoric acid gradient elution;flow rate of 0.4mL·min-1,detection wavelength of 327nm and 240nm,column temperature of 25℃,injection volume of 10μL.RESULTS The linear relationship,precision and stability of the nine active components were all in line with the requirements of methodology,the average recovery rate was 101.3%~102.6%.CONCLUSION The method is simple,accurate and feasible,which can provide the basis for the quality control of Rendongteng Capsules.

UPLCRendongteng Capsulesquality controlcontent determination

徐英辉、申茹、麦艳珍、侯秋苑、郭玉岩

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惠州卫生职业技术学院药学与检验学院,广东惠州 516025

黑龙江中医药大学药学院,黑龙江哈尔滨 150040

超高效液相色谱 忍冬藤胶囊 质量控制 含量测定

2024

化学工程师
黑龙江省化工研究院

化学工程师

CSTPCD
影响因子:0.243
ISSN:1002-1124
年,卷(期):2024.38(12)