During many life processes such as replication,transcription,double-strand breaks repair and so on,double-stranded DNA will temporarily unwind and form single strand DNA(ssDNA).ssDNA may affect genomic stability and may also participate in the formation of non-B DNA structure,which in turn regulates and influences many key cellular processes.This review briefly describes the causes of the formation of single-stranded DNA,the structures containing single-stranded DNA and their possible functions in cells,and summarizes some high-throughput analysis techniques of single-stranded DNA,which provides the method inspiration for the subsequent ssDNA research and promotes the further development of ssDNA analysis techniques and methods.
关键词
单链DNA/R环/G-四链体/高通量测序
Key words
single strand DNA/R-loop/G-quadruplex/high-throughput sequencing
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