Protective Effect of Earthworm on Liver and Kidney Injury in Experimental Diabetic Nephropathy
To investigate the protective effect and mechanism of earthworm decoction on liver and kidney of di-abetic nephropathy(DN)rats induced by high glucose and high fat combined with streptozotocin(STZ).Sixty healthy SD rats were randomly divided into 6 groups:normal group,model group,high,medium and low dose group of earthworm decoction and positive control group,with 10 rats in each group.Daily monitoring of the overall health and well-being of each animal group is essential;Four weeks after the drug intervention,blood was taken from the abdominal aorta to measure the whole blood RBC,Plt,WBC,Hb counts,serum low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),total cholesterol(TC),triglycerides(TG),alanine aminotransferase(ALT),aspartate aminotransferase(AST),fasting blood glucose(FBG),blood creatinine(SCr),blood urea nitrogen(BUN),blood uric acid(UA)and other indicators.The right kidney of each group was taken and the kidney weight/body weight was measured.The left lobe of liver and left kidney of each group were taken,and the liver tissue and kidney tissue were observed after HE staining.WBC,RBC,RBC,PLT,LDL-C,HDL-C,TC,TG,BUN,UA,SCr,FBG counts of the rats in the model group rose obviously compared with normal control group,Compared with the model group(P＜0.05),the drug groups of earthworm were significantly reduced,and there was no statistically significant difference between the earthworm group and the benazepril group.Test results displayed that compared with model control group the Hb counts enhanced in the normal group,the counts enhanced in the earthworm groups.And tissue section results showed that medium and high doses of earthworm decoction and benazepril group could improve liver fibrosis in diabetic nephropathy rats.Degree renal mesangial stroma,molding and granular denaturation.Earthworm decoction can improve the liver and kidney lesions of DN rats,reduce the TG,TC,HDL-C,ALT,AST and other indicators of DN rats,and reduce the urinary protein level of DN rats.The mechanism is related to the improvement of animal blood glucose and lipid disorders,inhibition of inflammatory re-sponse,and protection of liver and kidney tissues.
万方数据
维普
