Experimental Study on the Endometrial Receptivity and Progesterone Production in Polycystic Ovary Syndrome Model Mice Treated with Xiaoqi Tiaojing Tang Combined with Clomiphene
To explore the experimental study of Xiaonang Tiaojing tang combined with Clomiphene on the endo-metrial receptivity and luteinizing hormone in polycystic ovary syndrome(PCOS)model mice.Fifty female mice were randomly divided into a blank group,a model group,a Western medicine control group,a traditional Chinese medicine treatment group,and a combination of Chinese and Western medicine treatment group,with 10 mice in each group.Except for the blank group,each membrane making mouse was used to prepare a PC OS model using de-hydroepiandrosterone(DHEA)combined with high-fat diet.The Western medicine control group,Chinese medi-cine treatment group,and Chinese Western medicine combined treatment group were treated with clomiphene,Xiao-nang Tiaojing Tang,and clomiphene combined with Xiaonang Tiaojing Tang by gavage,respectively.The blank group and model group were treated with equal volume of physiological saline by gavage intervention.Monitor the body weight and estrus cycle of mice daily.HE staining was used to observe the morphological changes in mouse o-varian tissue and measure the thickness of the endometrium.Enzyme linked immunosorbent assay(ELISA)was used to determine the content of luteinizing hormone(LH)in each group of mice.Compared with the blank group,the body weight of each membrane making mouse increased significantly(P＜0.05),the estrus cycle was disrup-ted,the granulosa cell layer of ovarian follicles became thinner,the number of atresia follicles and cystic follicles in-creased,and oocytes disappeared.The endometrium is significantly thickened(P＜0.05),and LH levels are signifi-cantly elevated(P＜0.05).Compared with the model group,mice in the Western medicine control group,Chinese medicine treatment group,and Chinese Western medicine combined treatment group all showed regular estrus cy-cles;The granulosa cell layer of the ovary thickens,and the number of atresia follicles and cystic follicles decreases,with visible oocytes and mature follicles;The body weight of the mice decreased significantly(P＜0.05),but there was no significant difference among the three groups;The LH levels and endometrial thickness of mice were signifi-cantly reduced(P＜0.01),and the combination of Chinese and Western medicine treatment group showed the greatest reduction(P＜0.05).There was no statistically significant difference between the Western medicine con-trol group and the Chinese medicine treatment group(P＞0.05).Xiaoqi Tiaojing Tang combined with Clomiphene improves the estrus cycle and ovarian function of PCOS mice,reduces endometrial thickness and LH levels,thereby improving endometrial receptivity and endocrine disorders in PCOS mice,and exerting therapeutic effects on PCOS.
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