Research Process on Saussurea eopygmaea Hand-Mazz Polysaccharides Extraction
The phenol-sulfuric acid method was used to determine the polysaccharide content,with the yield of polysaccharide as the response value so as to obtain the optimal process of polysaccharides extraction and alcohol precipitation from Saussurea eopygmaea Hand-Mazz.In addition,the single-factor experiment was used to determine the extraction factor level,and the orthogonal design method was used to optimize the extraction process.The best conditions were as follows:w(H2O)∶w(Saussurea eopygmaea Hand-Mazz)was 20∶1,extract three time(three hours every time);The optimum alcohol precipitation conditions were as flollows:volume fraction 80％ethanol,1∶6 volume ratio of concentrated solution and ethanol,and 15 min of ethanol settling time,and the optimal detection wavelength was 490 nm.It was found that polysaccharide content was 8.87％on average with a relative standard deviation(RSD)below 2％.The optimal parameter for the extraction and alcohol sinking process is stable and reliable,and the quantitative analysis method is accurate and reproducible for the Saussurea eopygmaea Hand-Mazz which provides reference data for the development and utilization.
Saussurea eopygmaea Hand-Mazzorthogonal designpolysaccharidesextraction process
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