Determination of Luteolin in Duyiwei Capsules by High Performance Liquid Chromatography-Diode Array Detector
The approach of high performance liquid chromatography-diode array detector(HPLC-DAD)was established to determine luteolin in Duyiwei capsules:HPLC is performed on a BDS HYPERSIL C18 column(4.6 mm×250 mm,5 μm),methanol 0.1 wt%phosphoric acid(50∶50,V∶V)is adopted as mobile phase,the flow rate is 1.0 mL/min,the column temperature is 35℃and the detection wavelength is 350 nm.The results showed that luteolin has a good linearity in the range of 0.1～100 μmol/L.The recoveries of this method were 97.67%～105.77%with a relative standard deviation(RSD)less than 3%at three standard solution concentrations of 1.0,5.0 and 10 μmol/L.The average content of luteolin in duyiwei capsule was 78.43 μg/g.This HPLC-DAD method is convenient,feasible,sensitive and stable so that it can be used for the content determination and quality control of luteolin in duyiwei capsule.
high performance liquid chromatography-diode array detector(HPLC-DAD)luteolinduyiweicondition optimizingcontent determination
万方数据
维普
