Studies on High Performance Liquid Chromatography Fingerprint of Kalimeris indica(L.)Sch-Bip Based on Cluster and Principal Component Analysis
The content of vanillic acid,vanillin,ferulic acid,apigenin,rhein,emodin and chrysophanol in Kalimeris indica(L.)Sch-Bip was determined by high performance liquid chromatography(HPLC).The quality control and evaluation based on the comparative studies of Kalimeris indica(L.)Sch-Bip collected in many places were performed by using Statistical Product and Service Solutions(SPSS)26.0 software for cluster and principal component analysis.This 13 common peaks were identified from HPLC fingerprint,of which 7 common components were qualitatively analyzed.Ten batches of medicinal materials can be grouped into two categories and five principal components were extracted with a cumulative variance contribution rate of 91.913%.The method for establishing the fingerprint of Kalimeris indica(L.)Sch-Bip is efficient and simple.