A gel chromatography-ultraviolet method for determining polyvinylpyrrolidone content was established,and it was proved to possess good exclusive feasibility.The detection wavelength is 205 nm on a polymer gel column with 0.1 mol/L sodium chloride as mobile phase and acetonitrile(7∶3,V∶V)as mobile phase.The mass concentration of polyvinylpyrrolidone shows a good linear relationship with peak area in the range of 0.1～1.5 mg/mL,and the linear correlation coefficient is 0.999 9.The recoveries are 99.3％～102.0％and relative standard deviation(RSD)is 0.35％～0.81％at high,medium and low concentrations,which can meet the determination requirements with good accuracy.This established gel chromatography method is accurate,simple and suitable for the determination of polyvinylpyrrolidone in different applications.
维普
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