Study on DNA Methylation Modification Analysis Based on Mass Spectrometry
As an important regulation mode in epigenetic modification,DNA methylation affects a series of biological processes in living systems by regulating gene expression.The chromatography tandem-mass spectrometry is an important technology to study DNA methylation modification.With delving into the biological functions of mammalian DNA methylation,more and more advanced approaches and instruments are used to study epigenetic modification.In order to carry out qualitative and quantitative analysis and detection of DNA modification,in addition to high performance liquid chromatography tandem mass spectrometry technology(HPLC-MS)integrating different kinds of quality analyzers,matrix-assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-ToF-MS)and gas chromatography tandem mass spectrometry(GC-MS)have also been developed and applied in this area,thus greatly expanding the methods for DNA methylation modification investigations.This paper summarizes the advancements of mass spectrometry technology for studying epigenetic DNA methylation modification,hoping to provide valuable research strategies for DNA methylation modification.
NETL
NSTL
万方数据
维普
