Hydrophobic Filter Paper-Based Gold Nanoflowers SERS Substrate for Highly Sensitive Detection of Melamine in Milk
Highly sensitive detection of melamine in milk was achieved by using sea urchin-like gold nanoflowers(GNFs)of hydrophobic filter paper substrate combined with surface-enhanced Raman scattering(SERS)technology.GNFs prepared by levodopa reduction possess many sharp protrusions and tips that can stimulate strong localized electromagnetic enhancement(EM),creating a large number of"hot spots"and improving the sensitivity of SERS detection.The use of hydrophobic filter paper as the substrate improves the uneven distribution of nanomaterials on the paper-based substrate and the lack of sensitivity.The sensitivity,stability and reproducibility of the SERS substrate were then evaluated.By quantifying melamine in spiked milk,the logarithm of melamine concentration in milk showed a good linear relationship with the SERS intensity in the range of 1×10-3～1×10-9 mol/L,and the limit of detection(LOD)was as low as 43 pmol/L,which was much lower than that of the similar detection methods.Moreover,the results of this method for detecting the actual samples were not significantly different from those detected by liquid chromatography(p＞0.05).Hydrophobic filter paper-based GNFs SERS substrate offers the advantages of high sensitivity,stability,reproducibility and practicality,providing a reference for the quantitative determination of melamine in other foodstuffs such as milk.
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