Isolation and Identification of Exosomes from Defatted Bovine Milk as A Research-Based Experiment
Exosome as a kind of extracellular vesicles,are widely distributed in body fluid,such as blood,urine and milk,etc.Exosomes can be used as disease diagnostic marker,drug delivery carrier,etc.,which is an emerging research area in chemistry.Herein,we designed a research-based experiment of isolation and identification of exosomes from defatted bovine milk.In this experiment,many chemical theories will be involved,such as salting-out and isoelectric point based method for protein separation,extraction of exosomes by polymer precipitation,BCA assay for quantification of protein,synthesis of nanoparticles,colorimetric method for identification of exosomes,etc.On the basis of"Chemical experiment teaching suggestions for chemistry majors"and"National standard for teaching quality of chemistry specialty",this experiment involves many secondary disciplines,such as inorganic chemistry,analytical chemistry,biochemistry,physical chemistry,material chemistry,etc.Through accomplishing this experiments,the undergraduates will enhance their basic operation skills,improve the scientific literacy and the abilities of scientific research,exploration,and trouble shooting.This experiment can work together to be a comprehensive experiment,or be divided into several parts for independent experiments,satisfying the different requirements of the experiment teaching purpose.
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